PAMAM-functionalized paper as a new substrate for the paper spray mass spectrometry measurement of proteins
Loading...
Issue Date
2023-04-28
Authors
Pereira, Igor
Monaghan, Joseph
Abruzzi, Lucas R.
Gill, Chris G.
License
Subject
Abstract
Paper surface functionalization with polyamidoamine (PAMAM) dendrimers has been developed for increased sensitivity analysis of proteins by paper spray mass spectrometry (PS-MS). PAMAM is a branched polymeric compound with an ethylenediamine core linked to repeating PAMAM units that generates an outer surface rich in primary amines. These positively charged amine groups can interact electrostatically with negatively charged residues (e.g., aspartate, glutamate) on the protein surface. PAMAM inner amide moieties can also promote hydrogen bonding with protein surface oxygens, making PAMAM a useful material for protein extraction. PAMAM-functionalized PS-MS paper strips were used to extract proteins from biofluids, dipped in acetonitrile to remove unbound constituents, dried, and then measured with PS-MS. The use of this strategy was optimized and compared with unmodified paper strips. PAMAM-functionalized paper substrates provided sixfold greater sensitivity for albumin, 11-fold for hemoglobin, sevenfold for insulin, and twofold for lysozyme. The analytical performance of the functionalized paper substrate was evaluated through the analysis of albumin in urine, achieving linearity with R2 > 0.99, LOD of 1.1 μg mL–1, LOQ of 3.8 μg mL–1, precision better than 10%, and relative recovery 70–83%. The method was applied to quantify urinary albumin from nine anonymous patient samples (concentrations ranged from 6.5 to 77.4 μg mL–1), illustrating its potential for the diagnosis of microalbuminuria. These data demonstrate the utility of paper modification with the PAMAM dendrimer for sensitive PS-MS analysis of proteins, opening a path for further applications in clinical diagnosis through the analysis of disease-related proteins.
Description
This is the Accepted Manuscript of an article that was published as: Pereira, I., Monaghan, J., Abruzzi, L.R., & Gill, C.G. (2023). PAMAM-functionalized paper as a new substrate for the paper spray mass spectrometry measurement of proteins. Analytical Chemistry, 95(18), 7134–7141. https://doi.org/10.1021/acs.analchem.2c05316