Lab-on-a-chip technology for a non-invasive and real-time visualisation of metabolic activities in larval vertebrates

dc.contributor.authorZhu, Feng
dc.contributor.authorBaker, Daniel W.
dc.contributor.authorSkommer, Joanna
dc.contributor.authorSewell, Mary
dc.contributor.authorWlodkowic, Donald
dc.date.accessioned2017-06-02T18:04:28Z
dc.date.available2017-06-02T18:04:28Z
dc.date.issued2015-06-01
dc.description.abstractNon-invasive and real-time visualisation of metabolic activities in living small organisms such as zebrafish embryo and larvae has not yet been attempted due to profound analytical limitations of existing technologies. Significant progress in the development of physico-optical oxygen sensors using luminescence quenching by molecular oxygen has recently been made. Sensing using such microsensors is, however, still performed in small glass chambers that hold single specimens and thus not amenable for high-throughput data acquisition. In this work, we present a proof-of-concept approach by using microfluidic Lab-on-a-Chip (LOC) technologies combined with sophisticated optoelectronic sensors. The LOC device is capable of immobilising live zebrafish embryos with continuous flow perfusion, while the sensor uses innovative Fluorescence Ratiometric Imaging (FRIM) technology that can kinetically quantify the temporal patterns of aqueous oxygen gradients at a very fine scale based on signals coming from an optical sensor referred to as a sensor foil. By embedding the sensor foil onto the microfluidic living embryo array system, we demonstrated in situ FRIM on developing zebrafish embryos. Future integration of microfluidic chip-based technologies with FRIM technology represents a noteworthy direction to miniaturise and revolutionise research on metabolism and physiology in vivo.en
dc.description.fulltexthttps://viuspace.viu.ca/bitstream/handle/10613/4977/Baker.SPIE.9518.pdf?sequence=4en
dc.description.noteConference Proceedings: Bio-MEMS and Medical Microdevices II. Conference Date: May 5-6, 2015. Location: Barcelona, Spain.en
dc.description.noteThis is an electronic copy of a paper that originally appeared as: Zhu, F., Baker, D., Skommer, J., Sewell, M., & Wlodkowic, D. (2015). Lab-on-a-chip technology for a non-invasive and real-time visualization of metabolic activities in larval vertebrates. In S. van den Driesche (ed.), SPIE Proceedings 9518: Bio-MEMS and Medical Microdevices II, (pp. 951813-1–951813-6). Bellingham, WA: SPIE. DOI: 10.1117/12.2180690 Copyright 2015 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic electronic or print reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/12.2180690en
dc.description.noteArticle Number: 951813en
dc.format.extent6 p.en
dc.format.mediumtexten
dc.format.mimetypeapplication/pdfen
dc.identifier.citationZhu, F., Baker, D., Skommer, J., Sewell, M., & Wlodkowic, D. (2015). Lab-on-a-chip technology for a non-invasive and real-time visualization of metabolic activities in larval vertebrates. In S. van den Driesche (ed.), SPIE Proceedings 9518: Bio-MEMS and Medical Microdevices II, (pp. 951813-1–951813-6). Bellingham, WA: SPIE. DOI: 10.1117/12.2180690en
dc.identifier.isbn9781628416411
dc.identifier.issn1605-7422
dc.identifier.otherDOI: 10.1117/12.2180690en
dc.identifier.urihttp://hdl.handle.net/10613/4977
dc.language.isoenen
dc.publisherSPIEen
dc.relation.ispartofseriesProgress in biomedical optics and imaging ; volume 16, no. 50.en
dc.subject.lcshZebra danioen
dc.subject.lcshZebra danios as laboratory animalsen
dc.subject.lcshAquatic organisms--Monitoringen
dc.subject.lcshFishes--Embryosen
dc.subject.lcshFishes--Metabolismen
dc.subject.otherZebrafishen
dc.subject.otherLab-on-a-chip (LOC) technologiesen
dc.subject.otherFluorescence ratiometric imaging (FRIM) technologyen
dc.titleLab-on-a-chip technology for a non-invasive and real-time visualisation of metabolic activities in larval vertebratesen
dc.typeArticleen
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